Microfluidic devices for X-ray studies on hydrated cells
2013-01-21 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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- Authors
- Weinhausen, Britta; Köster, Sarah
- Abstract
- X-ray studies of biological cells in microfluidic devices provide a method to probe cellular structures or structural changes at the molecular level in a precisely controlled environment. However, the device design and the used materials must be compatible with X-ray scattering techniques as well as the cell culture in the devices. For this purpose, we develop new types of X-ray compatible microfluidic devices, which are based on a UV-curable adhesive as a moldable material, and thin Kapton films and silicon nitride membrane windows as a growth substrate for cells and as a window material for X-rays. Using these devices, we perform scanning X-ray diffraction experiments with a nano-focused beam on fixed cells in buffer solution. In principle, these microfluidic devices also allow for X-ray studies on living cells.
- Issue Date
- 21-January-2013
- Journal
- Lab on a Chip
- Organization
- Institut für Röntgenphysik
- Working Group
- RG Köster (Cellular Biophysics)
- ISSN
- 1473-0197
- Language
- English
- Subject(s)
- x-ray imaging; x-ray scattering; cellular biophysics; microfluidics