High-Fat Diet Induced Isoform Changes of the Parkinson’s Disease Protein DJ-1
2014 | journal article
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High-Fat Diet Induced Isoform Changes of the Parkinson’s Disease Protein DJ-1
Poschmann, G.; Seyfarth, K.; Besong Agbo, D.; Klafki, H.-W. ; Rozman, J.; Wurst, W. & Wiltfang, J. et al. (2014)
Journal of Proteome Research, 13(5) pp. 2339-2351. DOI: https://doi.org/10.1021/pr401157k
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- Authors
- Poschmann, Gereon; Seyfarth, Katrin; Besong Agbo, Daniela; Klafki, Hans-Wolfgang ; Rozman, Jan; Wurst, Wolfgang; Wiltfang, Jens ; Meyer, Helmut E.; Klingenspor, Martin; Stühler, Kai
- Abstract
- Genetic and environmental factors mediate via different physiological and molecular processes a shifted energy balance leading to overweight and obesity. To get insights into the underlying processes involved in energy intake and weight gain, we compared hypothalamic tissue of mice kept on a high-fat or control diet for 10 days by a proteomic approach. Using two-dimensional difference gel electrophoresis in combination with LC–MS/MS, we observed significant abundance changes in 15 protein spots. One isoform of the protein DJ-1 was elevated in the high-fat diet group in three different mouse strains SWR/J, C57BL/6N, and AKR/J analyzed. Large-scale validation of DJ-1 isoforms in individual samples and tissues confirmed a shift in the pattern of DJ-1 isoforms toward more acidic isoforms in several brain and peripheral tissues after feeding a high-fat diet for 10 days. The identification of oxidation of cysteine 106 as well as 2-succinyl modification of the same residue by mass spectrometry not only explains the isoelectric shift of DJ-1 but also links our results to similar shifts of DJ-1 observed in neurodegenerative disease states under oxidative stress. We hypothesize that DJ-1 is a common physiological sensor involved in both nutrition-induced effects and neurodegenerative disease states.
- Issue Date
- 2014
- Journal
- Journal of Proteome Research
- ISSN
- 1535-3893
- Language
- English