Phase-contrast x-ray imaging and tomography of the nematode Caenorhabditis elegans
2012-08-21 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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Phase-contrast x-ray imaging and tomography of the nematode Caenorhabditis elegans
Olendrowitz, C.; Bartels, M. ; Krenkel, M.; Beerlink, A. ; Mokso, R.; Sprung, M. & Salditt, T. (2012)
Physics in Medicine and Biology, 57(16). DOI: https://doi.org/10.1088/0031-9155/57/16/5309
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Details
- Authors
- Olendrowitz, Christian; Bartels, Matthias ; Krenkel, Martin; Beerlink, André ; Mokso, R.; Sprung, Michael; Salditt, Tim
- Abstract
- We have analyzed the model organism Caenorhabditis elegans with the help of phase-contrast x-ray tomography. This work combines techniques from x-ray imaging studies of single biological cells by in-line holography with three-dimensional reconstruction and furthermore extends these studies to the multicellular level. To preserve the sub-cellular ultrastructure of the nematodes, we used the near-native sample preparation of high-pressure freezing as commonly used in the field of electron microscopy. For the presented samples, a standard, non-magnifying parallel-beam setting, as well as a magnifying, divergent-beam setting using nanofocusing optics is evaluated based on their tomographic reconstruction potential. In this paper, we address difficulties in sample preparation and issues of image processing. By experimental refinement and through optimized reconstruction procedures, we were able to perform x-ray imaging studies on a living specimen.
- Issue Date
- 21-August-2012
- Journal
- Physics in Medicine and Biology
- Organization
- Institut für Röntgenphysik
- Working Group
- RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)
- ISSN
- 0031-9155
- Language
- English
- Subject(s)
- x-ray imaging; biomedical tomography