Peroxisomes contribute to intracellular calcium dynamics in cardiomyocytes and non-excitable cells
2021 | journal article. A publication with affiliation to the University of Göttingen.
Jump to: Cite & Linked | Documents & Media | Details | Version history
Cite this publication
Peroxisomes contribute to intracellular calcium dynamics in cardiomyocytes and non-excitable cells
Sargsyan, Y.; Bickmeyer, U.; Gibhardt, C. S; Streckfuss-Bömeke, K. ; Bogeski, I. & Thoms, S. (2021)
Life Science Alliance, 4(9) pp. e202000987. DOI: https://doi.org/10.26508/lsa.202000987
Documents & Media
Details
- Authors
- Sargsyan, Yelena; Bickmeyer, Uta; Gibhardt, Christine S; Streckfuss-Bömeke, Katrin ; Bogeski, Ivan ; Thoms, Sven
- Abstract
- Peroxisomes communicate with other cellular compartments by transfer of various metabolites. However, whether peroxisomes are sites for calcium handling and exchange has remained contentious. Here we generated sensors for assessment of peroxisomal calcium and applied them for single cell-based calcium imaging in HeLa cells and cardiomyocytes. We found that peroxisomes in HeLa cells take up calcium upon depletion of intracellular calcium stores and upon calcium influx across the plasma membrane. Furthermore, we show that peroxisomes of neonatal rat cardiomyocytes and human induced pluripotent stem cell–derived cardiomyocytes can take up calcium. Our results indicate that peroxisomal and cytosolic calcium signals are tightly interconnected both in HeLa cells and in cardiomyocytes. Cardiac peroxisomes take up calcium on beat-to-beat basis. Hence, peroxisomes may play an important role in shaping cellular calcium dynamics of cardiomyocytes.
Peroxisomes communicate with other cellular compartments by transfer of various metabolites. However, whether peroxisomes are sites for calcium handling and exchange has remained contentious. Here we generated sensors for assessment of peroxisomal calcium and applied them for single cell-based calcium imaging in HeLa cells and cardiomyocytes. We found that peroxisomes in HeLa cells take up calcium upon depletion of intracellular calcium stores and upon calcium influx across the plasma membrane. Furthermore, we show that peroxisomes of neonatal rat cardiomyocytes and human induced pluripotent stem cell–derived cardiomyocytes can take up calcium. Our results indicate that peroxisomal and cytosolic calcium signals are tightly interconnected both in HeLa cells and in cardiomyocytes. Cardiac peroxisomes take up calcium on beat-to-beat basis. Hence, peroxisomes may play an important role in shaping cellular calcium dynamics of cardiomyocytes. - Issue Date
- 2021
- Journal
- Life Science Alliance
- Project
- SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente
SFB 1190 | P17: Die Rolle mitochondrialer Kontaktstellen im Rahmen tumorrelevanter Calcium- und Redox-Signalwege
SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz
SFB 1002 | A02: Bedeutung des Phosphatase-Inhibitors-1 für die SR-spezifische Modulation der Beta- adrenozeptor-Signalkaskade - Working Group
- RG Bogeski
RG Thoms (Biochemistry and Molecular Medicine) - eISSN
- 2575-1077
- Language
- English