Reversible photoswitching enables single-molecule fluorescence fluctuation Spectroscopy at high molecular concentration
2007 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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Reversible photoswitching enables single-molecule fluorescence fluctuation Spectroscopy at high molecular concentration
Eggeling, C. ; Hilbert, M. ; Bock, H. ; Ringemann, C.; Hofmann, M.; Stiel, A. C. & Andresen, M. et al. (2007)
Microscopy research and technique, 70(12) pp. 1003-1009. DOI: https://doi.org/10.1002/jemt.20505
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Details
- Authors
- Eggeling, C. ; Hilbert, M. ; Bock, H. ; Ringemann, C.; Hofmann, M.; Stiel, A. C. ; Andresen, M. ; Jakobs, S. ; Egner, A. ; Schönle, A. ; Hell, S. W.
- Abstract
- We demonstrate that photoswitchable markers enable fluorescence fluctuation spectroscopy at high molecular concentration. Reversible photoswitching allows precise control of the density of fluorescing entities, because the equilibrium between the fluorescent ON- and the dark OFF-state can be shifted through optical irradiation at a specific wavelength. Depending on the irradiation intensity, the concentration of the ON-state markers can be up to 1,000 times lower than the actual concentration of the labeled molecular entity. Photoswitching expands the range of single-molecule detection based experiments such as fluorescence fluctuation spectroscopy to large entity concentrations in the micromolar range.
- Issue Date
- 2007
- Journal
- Microscopy research and technique
- ISSN
- 1059-910X
- Language
- English
