Active membranes studied by X-ray scattering
2007-08-22 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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- Authors
- Giahi, A.; Faris, M. El Alaoui; Bassereau, Patricia; Salditt, Tim
- Abstract
- In view of recent theories of "active" membranes, we have studied multilamellar phospholipid membrane stacks with reconstituted transmembrane protein bacteriorhodopsin (BR) under different illumination conditions by X-ray scattering. The light-active protein is considered as an active constituent which drives the system out of equilibrium and is predicted to change the collective fluctuation properties of the membranes. Using X-ray reflectivity, X-ray non-specular (diffuse) scattering, and grazing incidence scattering, we find no detectable change in the scattering curves when changing the illumination condition. In particular the intermembrane spacing d remains constant, after eliminating hydration-related artifacts by design of a suitable sample environment. The absence of any observable non-equilibrium effects in the experimental window is discussed in view of the relevant parameters and recent theories.
- Issue Date
- 22-August-2007
- Journal
- The European Physical Journal E - Soft Matter
- Organization
- Institut für Röntgenphysik
- Working Group
- RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)
- ISSN
- 1292-8941
- Subject(s)
- x-ray scattering; membrane biophysics