Characterization of the Munc13-calmodulin interaction by photoaffinity labeling
2006 | conference paper; research paper. A publication with affiliation to the University of Göttingen.
Jump to: Cite & Linked | Documents & Media | Details | Version history
Cite this publication
Characterization of the Munc13-calmodulin interaction by photoaffinity labeling
Dimova, K.; Kawabe, H.; Betz, A. ; Brose, N. & Jahn, O. (2006)
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. (11) (Vol. 1763). , Ecole Superieure Biotechnol, Strasbourg, FRANCE.
Amsterdam: Elsevier Science Bv. DOI: https://doi.org/10.1016/j.bbamcr.2006.09.017
Documents & Media
Details
- Authors
- Dimova, K.; Kawabe, H.; Betz, A. ; Brose, N. ; Jahn, O.
- Abstract
- Sensing of and response to transient increases in the residual presynaptic Ca2+ levels are important adaptive mechanisms that define the short-term plasticity characteristics of neurons. Due to their essential function in synaptic vesicle priming and in the modulation of synaptic strength, Munc13 proteins have emerged as key regulators of these adaptive mechanisms. Indeed, Munc13-1 and ubMunc13-2 contain a conserved calmodulin (CaM) binding site and the Ca2+-dependent interaction of these Munc 13 isoforms with CaM constitutes a molecular mechanism that transduces residual Ca2+ signaling to the synaptic exocytotic machinery. Here, we used Munc13-derived model peptides in photoaffinity labeling (PAL) experiments to demonstrate the stoichiometric and Ca2+-dependent CaM binding of the other members of the Munc13 family, bMunc13-2 and Munc13-3, via structurally distinct non-conserved binding sites. A PAL-based Ca2+ titration assay revealed that all Munc 13 isoforms can form a complex with CaM already at low Ca2+ concentrations just above resting levels, underscoring the Ca2+ sensor/effector function of this interaction in short-term synaptic plasticity phenomena. (c) 2006 Elsevier B.V. All rights reserved.
- Issue Date
- 2006
- Publisher
- Elsevier Science Bv
- Conference Place
- Ecole Superieure Biotechnol, Strasbourg, FRANCE
- ISSN
- 0167-4889