ATUM-FIB microscopy for targeting and multiscale imaging of rare events in mouse cortex
2020 | journal article; research paper
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- Authors
- Kislinger, Georg; Gnägi, Helmut; Kerschensteiner, Martin ; Simons, Mikael ; Misgeld, Thomas ; Schifferer, Martina
- Abstract
- Here, we describe a detailed workflow for ATUM-FIB microscopy, a hybrid method that combines serial-sectioning scanning electron microscopy (SEM) with focused ion beam SEM (FIB-SEM). This detailed protocol is optimized for mouse cortex samples. The main processing steps include the generation of semi-thick sections from sequentially cured resin blocks using a heated microtomy approach. We demonstrate the different imaging modalities, including serial light and electron microscopy for target recognition and FIB-SEM for isotropic imaging of regions of interest. For complete details on the use and execution of this protocol, please refer to Kislinger et al. (2020).
- Issue Date
- 2020
- Journal
- STAR Protocols
- Project
- TRR 274: Checkpoints of Central Nervous System Recovery
TRR 274 | B03: Checkpoints of recovery after primary astrocytic lesions in neuromyelitis optica and related animal models
TRR 274 | C02: In vivo detection and targeting of calcium clearance and axonal membrane repair after acute CNS insults
TRR 274 | Z01: Bioimaging Platform - Working Group
- RG Kerschensteiner (Neuroimmune Interactions)
RG Misgeld
RG Schifferer
RG Simons (The Biology of Glia in Development and Disease) - eISSN
- 2666-1667
- Language
- English