The conserved protein Seb1 drives transcription termination by binding RNA polymerase II and nascent RNA
2017 | journal article
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The conserved protein Seb1 drives transcription termination by binding RNA polymerase II and nascent RNA
Wittmann, S.; Renner, M.; Watts, B. R.; Adams, O.; Huseyin, M.; Baejen, C. & El Omari, K. et al. (2017)
Nature Communications, 8 art. 14861. DOI: https://doi.org/10.1038/ncomms14861
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Details
- Authors
- Wittmann, Sina; Renner, Max; Watts, Beth R.; Adams, Oliver; Huseyin, Miles; Baejen, Carlo; El Omari, Kamel; Kilchert, Cornelia; Heo, Dong-Hyuk; Kecman, Tea; Cramer, Patrick ; Grimes, Jonathan M.; Vasiljeva, Lidia
- Abstract
- Termination of RNA polymerase II (Pol II) transcription is an important step in the transcription cycle, which involves the dislodgement of polymerase from DNA, leading to release of a functional transcript. Recent studies have identified the key players required for this process and showed that a common feature of these proteins is a conserved domain that interacts with the phosphorylated C-terminus of Pol II (CTD-interacting domain, CID). However, the mechanism by which transcription termination is achieved is not understood. Using genome-wide methods, here we show that the fission yeast CID-protein Seb1 is essential for termination of protein-coding and non-coding genes through interaction with S2-phosphorylated Pol II and nascent RNA. Furthermore, we present the crystal structures of the Seb1 CTD- and RNA-binding modules. Unexpectedly, the latter reveals an intertwined two-domain arrangement of a canonical RRM and second domain. These results provide important insights into the mechanism underlying eukaryotic transcription termination.
- Issue Date
- 2017
- Journal
- Nature Communications
- eISSN
- 2041-1723
- Language
- English