Vimentin intermediate filaments structure and mechanically support microtubules in cells
2023-04-21 | preprint. A publication with affiliation to the University of Göttingen.
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- Authors
- Blob, Anna; Ventzke, David; Nies, Giacomo; Dühmert, Jan Niklas; Schmitzer, Bernhard ; Munk, Axel ; Schaedel, Laura; Köster, Sarah
- Abstract
- The eukaryotic cytoskeleton comprises three types of mechanically distinct biopolymers – actin filaments, microtubules and intermediate filaments (IFs)– along with passive crosslinkers and active molecular motors. Among these filament types, IFs are expressed in a cell-type specific manner and vimentin is found in cells of mesenchymal origin. The composite cytoskeletal network determines the mechanical and dynamic properties of the cell and is specifically governed by the interplay of the three different filament systems. We study the influence of vimentin IFs on the mechanics and network structure of microtubules by analyzing fluorescence micrographs of fibroblasts on protein micropatterns. We develop and apply quantitative, automated data analysis to a large number of cells, thus mitigating the considerable natural variance in data from biological cells. We find that the presence of a vimentin IF network structures and aligns microtubules in the cell interior. On a local scale, we observe higher microtubule curvatures when vimentin IFs are present, irrespective of whether the cells are polarized or not. Our results suggest that the vimentin IF network laterally supports microtubules against compressive buckling forces and further helps to structure the microtubule network, thus possibly leading to a more efficient intracellular transport system along the microtubules.
- Issue Date
- 21-April-2023
- Project
- SFB 1456 | Cluster A | A04: Dynamics of cytoskeletal networks: From geometric structure to cell mechanics
SFB 1456 | Cluster A: Data with Geometric Nonlinearities
SFB 1456: Mathematik des Experiments: Die Herausforderung indirekter Messungen in den Naturwissenschaften
EXC 2067: Multiscale Bioimaging - Organization
- Institut für Röntgenphysik
- Working Group
- RG Köster (Cellular Biophysics)
RG Munk - Extent
- 29
- Language
- English
- Fulltext
- https://www.biorxiv.org/content/10.1101/2023.04.19.537509v1.full.pdf
- Subject(s)
- cellular biophysics; cytoskeleton