High-resolution analysis of bound Ca 2+ in neurons and synapses
2023 | journal article. A publication with affiliation to the University of Göttingen.
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- Authors
- Bonnin, Elisa A.; Golmohammadi, Arash; Rehm, Ronja; Tetzlaff, Christian; Rizzoli, Silvio O.
- Abstract
- Calcium (Ca 2+ ) is a well-known second messenger in all cells, and is especially relevant for neuronal activity. Neuronal Ca 2+ is found in different forms, with a minority being freely soluble in the cell and more than 99% being bound to proteins. Free Ca 2+ has received much attention over the last few decades, but protein-bound Ca 2+ has been difficult to analyze. Here, we introduce correlative fluorescence and nanoscale secondary ion mass spectrometry imaging as a tool to describe bound Ca 2+ . As expected, bound Ca 2+ is ubiquitous. It does not correlate to free Ca 2+ dynamics at the whole-neuron level, but does correlate significantly to the intensity of markers for GABAergic pre-synapse and glutamatergic post-synapses. In contrast, a negative correlation to pre-synaptic activity was observed, with lower levels of bound Ca 2+ observed in the more active synapses. We conclude that bound Ca 2+ may regulate neuronal activity and should receive more attention in the future.
- Issue Date
- 2023
- Journal
- Life Science Alliance
- Project
- EXC 2067: Multiscale Bioimaging
SFB 1286: Quantitative Synaptologie
SFB 1286 | A03: Dynamische Analyse der Remodellierung der extrazellulären Matrix (ECM) als Mechanismus der Synapsenorganisation und Plastizität
SFB 1286 | C01: Die plastizitätsabhängige räumliche und zeitliche Organisation von AMPA-Rezeptoren und Gerüstproteinen - Working Group
- RG Rizzoli (Quantitative Synaptology in Space and Time)
RG Tetzlaff (Computational Neuroscience - Learning and Memory) - eISSN
- 2575-1077
- Language
- English
- Sponsor
- Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659
Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659