Synapsin condensation controls synaptic vesicle sequestering and dynamics
2023 | journal article. A publication with affiliation to the University of Göttingen.
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Synapsin condensation controls synaptic vesicle sequestering and dynamics
Hoffmann, C.; Rentsch, J.; Tsunoyama, T. A.; Chhabra, A.; Aguilar Perez, G.; Chowdhury, R. & Trnka, F. et al. (2023)
Nature Communications, 14(1). DOI: https://doi.org/10.1038/s41467-023-42372-6
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Details
- Authors
- Hoffmann, Christian; Rentsch, Jakob; Tsunoyama, Taka A.; Chhabra, Akshita; Aguilar Perez, Gerard; Chowdhury, Rajdeep; Trnka, Franziska; Korobeinikov, Aleksandr A.; Shaib, Ali H.; Ganzella, Marcelo; Milovanovic, Dragomir
- Abstract
- Abstract Neuronal transmission relies on the regulated secretion of neurotransmitters, which are packed in synaptic vesicles (SVs). Hundreds of SVs accumulate at synaptic boutons. Despite being held together, SVs are highly mobile, so that they can be recruited to the plasma membrane for their rapid release during neuronal activity. However, how such confinement of SVs corroborates with their motility remains unclear. To bridge this gap, we employ ultrafast single-molecule tracking (SMT) in the reconstituted system of native SVs and in living neurons. SVs and synapsin 1, the most highly abundant synaptic protein, form condensates with liquid-like properties. In these condensates, synapsin 1 movement is slowed in both at short (i.e., 60-nm) and long (i.e., several hundred-nm) ranges, suggesting that the SV-synapsin 1 interaction raises the overall packing of the condensate. Furthermore, two-color SMT and super-resolution imaging in living axons demonstrate that synapsin 1 drives the accumulation of SVs in boutons. Even the short intrinsically-disordered fragment of synapsin 1 was sufficient to restore the native SV motility pattern in synapsin triple knock-out animals. Thus, synapsin 1 condensation is sufficient to guarantee reliable confinement and motility of SVs, allowing for the formation of mesoscale domains of SVs at synapses in vivo.
- Issue Date
- 2023
- Journal
- Nature Communications
- Project
- SFB 1286: Quantitative Synaptologie
SFB 1286 | A03: Dynamische Analyse der Remodellierung der extrazellulären Matrix (ECM) als Mechanismus der Synapsenorganisation und Plastizität
SFB 1286 | B10: Nanoskalige Dynamik und Regulation von Synapsin-Kondensaten
EXC 2067: Multiscale Bioimaging - Working Group
- RG Milovanovic (Molecular Neuroscience)
RG Rizzoli (Quantitative Synaptology in Space and Time) - eISSN
- 2041-1723
- Language
- English