Longitudinal x-ray based lung function measurement for monitoring Nintedanib treatment response in a mouse model of lung fibrosis
2023 | journal article. A publication with affiliation to the University of Göttingen.
Jump to: Cite & Linked | Documents & Media | Details | Version history
Cite this publication
Longitudinal x-ray based lung function measurement for monitoring Nintedanib treatment response in a mouse model of lung fibrosis
Khan, A.; Markus, M. A.; Svetlove, A.; Hülsmann, S. ; Alves, F. & Dullin, C. (2023)
Scientific Reports, 13(1). DOI: https://doi.org/10.1038/s41598-023-45305-x
Documents & Media
Details
- Authors
- Khan, Amara; Markus, M. Andrea; Svetlove, Angelika; Hülsmann, Swen ; Alves, Frauke; Dullin, Christian
- Abstract
- Abstract Lung fibrosis (LF) is a chronic progressive, incurable, and debilitating condition of the lung, which is associated with different lung disease. Treatment options are still sparse. Nintedanib, an oral tyrosine kinase inhibitor, significantly slows the LF progression. However, there is a strong need of further research and the development of novel therapies. In this study, we used a correlative set-up that combines X-ray based lung function (XLF) with microCT and whole body plethysmography (WBP) for a comprehensive functional and structural evaluation of lung fibrosis (LF) as well as for monitoring response to orally administered Nintedanib in the mouse model of bleomycin induced LF. The decline in lung function as early as one week after intratracheal bleomycin instillation was reliably detected by XLF, revealing the lowest decay rate in the LF mice compared to healthy ones. Simultaneously performed microCT and WBP measurements corroborated XLF findings by exhibiting reduced lung volume $V^{insp}_{\mu CT}$ V μ C T insp and tidal volume $TV_{WBP}$ T V WBP . In LF mice XLF also revealed profound improvement in lung function one week after Nintedanib treatment. This positive response to Nintedanib therapy was further substantiated by microCT and WBP measurements which also showed significantly improved $V^{insp}_{\mu CT}$ V μ C T insp and $TV_{WBP}$ T V WBP in the Nintedanib treated mice. By comparing the XLF data to structural features assessing the extent of fibrosis obtained by ex-vivo high-resolution synchrotron radiation-based imaging and classical histology we demonstrate that: (1) a simple low dose x-ray measurement like XLF is sensitive enough to pick up treatment response, (2) Nintedanib treatment successfully improved lung function in a bleomycin induced LF mouse model and (3) differences between the fully restored lung function and the partially reduced fibrotic burden compared to healthy and untreated mice. The presented analysis pipeline underlines the importance of a combined functional and anatomical readout to reliably measure treatment response and could easily be adapted to other preclinical lung disease models.
- Issue Date
- 2023
- Journal
- Scientific Reports
- Project
- EXC 2067: Multiscale Bioimaging
- Working Group
- RG Alves (Translationale Molekulare Bildgebung)
- eISSN
- 2045-2322
- Language
- English