Rapid Identification of Genes Encoding DNA Polymerases by Function-Based Screening of Metagenomic Libraries Derived from Glacial Ice
2009 | journal article. A publication with affiliation to the University of Göttingen.
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Rapid Identification of Genes Encoding DNA Polymerases by Function-Based Screening of Metagenomic Libraries Derived from Glacial Ice
Simon, C.; Herath, J.; Rockstroh, S. & Daniel, R. (2009)
Applied and Environmental Microbiology, 75(9) pp. 2964-2968. DOI: https://doi.org/10.1128/AEM.02644-08
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Details
- Authors
- Simon, Carola; Herath, Judith; Rockstroh, Stephanie; Daniel, Rolf
- Abstract
- Small-insert and large-insert metagenomic libraries were constructed from glacial ice of the Northern Schneeferner, which is located on the Zugspitzplatt in Germany. Subsequently, these libraries were screened for the presence of DNA polymerase-encoding genes by complementation of an Escherichia coli polA mutant. Nine novel genes encoding complete DNA polymerase I proteins or domains typical of these proteins were recovered.
- Issue Date
- 2009
- Journal
- Applied and Environmental Microbiology
- ISSN
- 0099-2240
- Sponsor
- Bundesministerium fur Bildung und Forschung
