Functional interaction of the active zone proteins Munc13-1 and RIM1 in synaptic vesicle priming
2001 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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Functional interaction of the active zone proteins Munc13-1 and RIM1 in synaptic vesicle priming
Betz, A. ; Thakur, P.; Junge, H. J.; Ashery, U.; Rhee, J.-S. ; Scheuss, V. & Rosenmund, C. et al. (2001)
Neuron, 30(1) pp. 183-196. DOI: https://doi.org/10.1016/S0896-6273(01)00272-0
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- Authors
- Betz, Andrea ; Thakur, P; Junge, Harald J.; Ashery, Uri; Rhee, Jeong-Seop ; Scheuss, V.; Rosenmund, C.; Rettig, Jens; Brose, Nils
- Abstract
- Synaptic neurotransmitter release is restricted to active zones, where the processes of synaptic vesicle tethering, priming to fusion competence, and Ca2+-triggered fusion are taking place in a highly coordinated manner, We show that the active zone components Munc13-1, an essential vesicle priming protein, and RIM1, a Rab3 effector with a putative role in vesicle tethering, interact functionally. Disruption of this interaction causes a loss of fusion-competent synaptic vesicles, creating a phenocopy of Munc13-1-deficient neurons. RIM1 binding and Vesicle priming are mediated by two distinct structural modules of Munc13-1. The Munc13-1/RIM1 interaction may create a functional link between synaptic vesicle tethering and priming, or it may regulate the priming reaction itself, thereby determining the number of fusion-competent vesicles.
- Issue Date
- 2001
- Journal
- Neuron
- ISSN
- 0896-6273