Hybrid Structure of the Type 1 Pilus of Uropathogenic Escherichia coli
2015 | journal article. A publication with affiliation to the University of Göttingen.
Jump to: Cite & Linked | Documents & Media | Details | Version history
Cite this publication
Hybrid Structure of the Type 1 Pilus of Uropathogenic Escherichia coli
Habenstein, B. ; Loquet, A.; Hwang, S.; Giller, K. ; Vasa, S. K.; Becker, S. & Habeck, M. et al. (2015)
Angewandte Chemie International Edition, 54(40) pp. 11691-11695. DOI: https://doi.org/10.1002/anie.201505065
Documents & Media
Details
- Authors
- Habenstein, Birgit ; Loquet, Antoine; Hwang, Songhwan; Giller, Karin ; Vasa, Suresh Kumar; Becker, Stefan; Habeck, Michael ; Lange, Adam
- Abstract
- Type 1 pili are filamentous protein assemblies on the surface of Gram-negative bacteria that mediate adhesion to host cells during the infection process. The molecular structure of type 1 pili remains elusive on the atomic scale owing to their insolubility and noncrystallinity. Herein we describe an approach for hybrid-structure determination that is based on data from solution-state NMR spectroscopy on the soluble subunit and solid-state NMR spectroscopy and STEM data on the assembled pilus. Our approach is based on iterative modeling driven by structural information extracted from different sources and provides a general tool to access pseudo atomic structures of protein assemblies with complex subunit folds. By using this methodology, we determined the local conformation of the FimA pilus subunit in the context of the assembled type 1 pilus, determined the exact helical pilus architecture, and elucidated the intermolecular interfaces contributing to pilus assembly and stability with atomic detail.
- Issue Date
- 2015
- Status
- published
- Publisher
- Wiley-v C H Verlag Gmbh
- Journal
- Angewandte Chemie International Edition
- ISSN
- 1521-3773; 1433-7851