The Morphological and Molecular Nature of Synaptic Vesicle Priming at Presynaptic Active Zones
2014 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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The Morphological and Molecular Nature of Synaptic Vesicle Priming at Presynaptic Active Zones
Imig, C.; Min, S.-W.; Krinner, S. ; Arancillo, M.; Rosenmund, C.; Südhof, T. C. & Rhee, J. et al. (2014)
Neuron, 84(2) pp. 416-431. DOI: https://doi.org/10.1016/j.neuron.2014.10.009
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- Authors
- Imig, Cordelia; Min, Sang-Won; Krinner, Stefanie ; Arancillo, Marife; Rosenmund, Christian; Südhof, Thomas C.; Rhee, JeongSeop ; Brose, Nils ; Cooper, Benjamin H.
- Abstract
- Synaptic vesicle docking, priming, and fusion at active zones are orchestrated by a complex molecular machinery. We employed hippocampal organotypic slice cultures from mice lacking key presynaptic proteins, cryofixation, and three-dimensional electron tomography to study the mechanism of synaptic vesicle docking in the same experimental setting, with high precision, and in a near-native state. We dissected previously indistinguishable, sequential steps in synaptic vesicle active zone recruitment (tethering) and membrane attachment (docking) and found that vesicle docking requires Munc13/CAPS family priming proteins and all three neuronal SNAREs, but not Synaptotagmin-1 or Complexins. Our data indicate that membrane-attached vesicles comprise the readily releasable pool of fusion-competent vesicles and that synaptic vesicle docking, priming, and trans-SNARE complex assembly are the respective morphological, functional, and molecular manifestations of the same process, which operates downstream of vesicle tethering by active zone components.
- Issue Date
- 2014
- Journal
- Neuron
- ISSN
- 0896-6273
- eISSN
- 1097-4199