Anti-CSF-1 treatment is effective to prevent carcinoma invasion induced by monocyte-derived cells but scarcely by microglia.
2015-06-20 | journal article. A publication with affiliation to the University of Göttingen.
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Anti-CSF-1 treatment is effective to prevent carcinoma invasion induced by monocyte-derived cells but scarcely by microglia.
Rietkötter, E.; Bleckmann, A.; Bayerlová, M.; Menck, K.; Chuang, H.-N.; Wenske, B. & Schwartz, H. et al. (2015)
Oncotarget, 6(17) pp. 15482-15493. DOI: https://doi.org/10.18632/oncotarget.3855
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Details
- Authors
- Rietkötter, Eva; Bleckmann, Annalen; Bayerlová, Michaela; Menck, Kerstin; Chuang, Han-Ning; Wenske, Britta; Schwartz, Hila; Erez, Neta; Binder, Claudia; Hanisch, Uwe-Karsten; Pukrop, Tobias
- Abstract
- The mononuclear phagocytic system is categorized in three major groups: monocyte-derived cells (MCs), dendritic cells and resident macrophages. During breast cancer progression the colony stimulating factor 1 (CSF-1) can reprogram MCs into tumor-promoting macrophages in the primary tumor. However, the effect of CSF-1 during colonization of the brain parenchyma is largely unknown. Thus, we analyzed the outcome of anti-CSF-1 treatment on the resident macrophage population of the brain, the microglia, in comparison to MCs, alone and in different in vitro co-culture models. Our results underline the addiction of MCs to CSF-1 while surprisingly, microglia were not affected. Furthermore, in contrast to the brain, the bone marrow did not express the alternative ligand, IL-34. Yet treatment with IL-34 and co-culture with carcinoma cells partially rescued the anti-CSF-1 effects on MCs. Further, MC-induced invasion was significantly reduced by anti-CSF-1 treatment while microglia-induced invasion was reduced to a lower extend. Moreover, analysis of lung and breast cancer brain metastasis revealed significant differences of CSF-1 and CSF-1R expression. Taken together, our findings demonstrate not only differences of anti-CSF-1 treatment on MCs and microglia but also in the CSF-1 receptor and ligand expression in brain and bone marrow as well as in brain metastasis.
- Issue Date
- 20-June-2015
- Journal
- Oncotarget
- ISSN
- 1949-2553
- Language
- English