Activin A increases phagocytosis of Escherichia coli K1 by primary murine microglial cells activated by toll-like receptor agonists
2018 | journal article. A publication with affiliation to the University of Göttingen.
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Activin A increases phagocytosis of Escherichia coli K1 by primary murine microglial cells activated by toll-like receptor agonists
Diesselberg, C.; Ribes, S.; Seele, J.; Kaufmann, A.; Redlich, S.; Bunkowski, S. & Hanisch, U.-K. et al. (2018)
Journal of Neuroinflammation, 15(1) art. 175. DOI: https://doi.org/10.1186/s12974-018-1209-2
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- Authors
- Diesselberg, Catharina; Ribes, Sandra; Seele, Jana; Kaufmann, Annika; Redlich, Sandra; Bunkowski, Stephanie; Hanisch, Uwe-Karsten; Michel, Uwe; Nau, Roland; Schütze, Sandra
- Abstract
- BACKGROUND: Bacterial meningitis is associated with high mortality and long-term neurological sequelae. Increasing the phagocytic activity of microglia could improve the resistance of the CNS against infections. We studied the influence of activin A, a member of the TGF-β family with known immunoregulatory and neuroprotective effects, on the functions of microglial cells in vitro. METHODS: Primary murine microglial cells were treated with activin A (0.13 ng/ml-13 μg/ml) alone or in combination with agonists of TLR2, 4, and 9. Phagocytosis of Escherichia coli K1 as well as release of TNF-α, IL-6, CXCL1, and NO was assessed. RESULTS: Activin A dose-dependently enhanced the phagocytosis of Escherichia coli K1 by microglial cells activated by agonists of TLR2, 4, and 9 without further increasing NO and proinflammatory cytokine release. Cell viability of microglial cells was not affected by activin A. CONCLUSIONS: Priming of microglial cells with activin A could increase the elimination of bacteria in bacterial CNS infections. This preventive strategy could improve the resistance of the brain to infections, particularly in elderly and immunocompromised patients.
- Issue Date
- 2018
- Journal
- Journal of Neuroinflammation
- Organization
- Institut für Neuropathologie ; Klinik für Neurologie
- Language
- English