The target of the DEAH-box NTP triphosphatase Prp43 in Saccharomyces cerevisiae spliceosomes is the U2 snRNP-intron interaction
2016 | journal article. A publication with affiliation to the University of Göttingen.
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The target of the DEAH-box NTP triphosphatase Prp43 in Saccharomyces cerevisiae spliceosomes is the U2 snRNP-intron interaction
Fourmann, J.-B.; Dybkov, O.; Agafonov, D. E; Tauchert, M. J ; Urlaub, H. ; Ficner, R. & Fabrizio, P. et al. (2016)
eLife, 5 art. e15564. DOI: https://doi.org/10.7554/eLife.15564
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- Authors
- Fourmann, Jean-Baptiste; Dybkov, Olexandr; Agafonov, Dmitry E; Tauchert, Marcel J ; Urlaub, Henning ; Ficner, Ralf ; Fabrizio, Patrizia; Lührmann, Reinhard
- Abstract
- The DEAH-box NTPase Prp43 and its cofactors Ntr1 and Ntr2 form the NTR complex and are required for disassembling intron-lariat spliceosomes (ILS) and defective earlier spliceosomes. However, the Prp43 binding site in the spliceosome and its target(s) are unknown. We show that Prp43 fused to Ntr1's G-patch motif (Prp43_Ntr1GP) is as efficient as the NTR in ILS disassembly, yielding identical dissociation products and recognizing its natural ILS target even in the absence of Ntr1's C-terminal-domain (CTD) and Ntr2. Unlike the NTR, Prp43_Ntr1GP disassembles earlier spliceosomal complexes (A, B, B(act)), indicating that Ntr2/Ntr1-CTD prevents NTR from disrupting properly assembled spliceosomes other than the ILS. The U2 snRNP-intron interaction is disrupted in all complexes by Prp43_Ntr1GP, and in the spliceosome contacts U2 proteins and the pre-mRNA, indicating that the U2 snRNP-intron interaction is Prp43's major target.
- Issue Date
- 2016
- Journal
- eLife
- eISSN
- 2050-084X
- ISSN
- 2050-084X
- Language
- English