Specific 14-3-3 isoform detection and immunolocalization in prion diseases
2002 | journal article. A publication with affiliation to the University of Göttingen.
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Specific 14-3-3 isoform detection and immunolocalization in prion diseases
Baxter, H. C.; Fraser, J. R.; Liu, W.-G.; Forster, J. L.; Clokie, S.; Steinacker, P. & Otto, M. et al. (2002)
Biochemical Society Transactions, 30(4) pp. 387-391. DOI: https://doi.org/10.1042/bst0300387
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Details
- Authors
- Baxter, H. C.; Fraser, J. R.; Liu, W.-G.; Forster, J. L.; Clokie, S.; Steinacker, P. ; Otto, M. ; Bahn, E. ; Wiltfang, J. ; Aitken, A.
- Abstract
- 14-3-3 proteins are involved in signalling processes in neuronal cells. Using isoform-specific antibodies we have examined the variation in 14-3-3 isoform neurolocation in normal and scrapie-infected murine brain and show that in defined areas of the brain there are significant changes associated with the pathology of the disease process. The appearance of 14-3-3 proteins in the cerebrospinal fluid (CSF) is a consequence of neuronal disease and the detection of specific isoforms of the 14-3-3 proteins in the CSF is characteristic of some neurodegenerative diseases. In this study, monitoring specifically for theγ 14-3-3 isoform in the CSF by both Western-blot analysis and ELISA we can show a level of correlation between the assays.
- Issue Date
- 2002
- Journal
- Biochemical Society Transactions
- ISSN
- 0300-5127
- Language
- English
