Multicolour Multilevel STED nanoscopy of Actin/Spectrin Organization at Synapses
2016 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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- Authors
- Sidenstein, Sven C. ; D'Este, Elisa ; Böhm, Marvin J.; Danzl, Johann G. ; Belov, Vladimir N. ; Hell, Stefan
- Abstract
- Superresolution fluorescence microscopy of multiple fluorophores still requires development. Here we present simultaneous three-colour stimulated emission depletion (STED) nanoscopy relying on a single STED beam at 620 nm. Toggling the STED beam between two or more power levels ("multilevelSTED") optimizes resolution and contrast in all colour channels, which are intrinsically co-aligned and well separated. Three-colour recording is demonstrated by imaging the nanoscale cytoskeletal organization in cultured hippocampal neurons. The down to similar to 35 nm resolution identified periodic actin/betaII spectrin lattices along dendrites and spines; however, at presynaptic and postsynaptic sites, these patterns were found to be absent. Both our multicolour scheme and the 620 nm STED line should be attractive for routine STED microscopy applications.
- Issue Date
- 2016
- Journal
- Scientific Reports
- ISSN
- 2045-2322
- Language
- English