Gold-Conjugated Nanobodies for Targeted Imaging Using High-Resolution Secondary Ion Mass Spectrometry
2021 | journal article. A publication with affiliation to the University of Göttingen.
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- Authors
- Agüi-Gonzalez, Paola ; Dankovich, Tal M.; Rizzoli, Silvio O. ; Phan, Nhu T. N.
- Abstract
- Nanoscale imaging with the ability to identify cellular organelles and protein complexes has been a highly challenging subject in the secondary ion mass spectrometry (SIMS) of biological samples. This is because only a few isotopic tags can be used successfully to target specific proteins or organelles. To address this, we generated gold nanoprobes, in which gold nanoparticles are conjugated to nanobodies. The nanoprobes were well suited for specific molecular imaging using NanoSIMS at subcellular resolution. They were demonstrated to be highly selective to different proteins of interest and sufficiently sensitive for SIMS detection. The nanoprobes offer the possibility of correlating the investigation of cellular isotopic turnover to the positions of specific proteins and organelles, thereby enabling an understanding of functional and structural relations that are currently obscure.
Nanoscale imaging with the ability to identify cellular organelles and protein complexes has been a highly challenging subject in the secondary ion mass spectrometry (SIMS) of biological samples. This is because only a few isotopic tags can be used successfully to target specific proteins or organelles. To address this, we generated gold nanoprobes, in which gold nanoparticles are conjugated to nanobodies. The nanoprobes were well suited for specific molecular imaging using NanoSIMS at subcellular resolution. They were demonstrated to be highly selective to different proteins of interest and sufficiently sensitive for SIMS detection. The nanoprobes offer the possibility of correlating the investigation of cellular isotopic turnover to the positions of specific proteins and organelles, thereby enabling an understanding of functional and structural relations that are currently obscure. - Issue Date
- 2021
- Journal
- Nanomaterials
- Project
- SFB 1286: Quantitative Synaptologie
SFB 1286 | A03: Dynamische Analyse der Remodellierung der extrazellulären Matrix (ECM) als Mechanismus der Synapsenorganisation und Plastizität
SFB 1286 | B01: Der Verteilung struktureller Lipide in synaptischen Membranen - Working Group
- RG Phan
RG Rizzoli (Quantitative Synaptology in Space and Time) - eISSN
- 2079-4991
- Language
- English
- Sponsor
- Deutsche Forschungsgemeinschaft
Vetenskapsrådet
Hasselblad Foundation