High-resolution reconstruction of the beating zebrafish heart
2014-09 | journal article; research paper
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- Authors
- Mickoleit, Michaela; Schmid, Benjamin; Weber, Michael; Fahrbach, Florian O.; Hombach, Sonja; Reischauer, Sven; Huisken, Jan
- Abstract
- The heart's continuous motion makes it difficult to capture high-resolution images of this organ in vivo. We developed tools based on high-speed selective plane illumination microscopy (SPIM), offering pristine views into the beating zebrafish heart. We captured three-dimensional cardiac dynamics with postacquisition synchronization of multiview movie stacks, obtained static high-resolution reconstructions by briefly stopping the heart with optogenetics and resolved nonperiodic phenomena by high-speed volume scanning with a liquid lens.
- Issue Date
- September-2014
- Journal
- Nature Methods
- ISSN
- 1548-7091
- eISSN
- 1548-7105
- Language
- English