A potassium channel protein encoded by chlorella virus PBCV-1
2000 | journal article. A publication with affiliation to the University of Göttingen.
Jump to: Cite & Linked | Documents & Media | Details | Version history
Cite this publication
Plugge, B., S. Gazzarrini, M. Nelson, R. Cerana, J. L. van Etten, C. Derst, D. DiFrancesco, A. Moroni, and G. Thiel. "A potassium channel protein encoded by chlorella virus PBCV-1." Science 287, no. 5458 (2000): 1641-1644. https://doi.org/10.1126/science.287.5458.1641.
Documents & Media
Details
- Authors
- Plugge, B.; Gazzarrini, S.; Nelson, M.; Cerana, R.; van Etten, J. L.; Derst, C.; DiFrancesco, D.; Moroni, A.; Thiel, G.
- Abstract
- The Large chlorella virus PBCV-1, which contains double-stranded DNA (dsDNA), encodes a 94-codon open reading frame (ORF) that contains a motif resembling the signature sequence of the pore domain of potassium channel proteins. Phylogenetic analyses of the encoded protein, Kcv, indicate a previously unidentified type of potassium channel. The messenger RNA encoded by the ORF Leads to functional expression of a potassium-selective conductance in Xenopus laevis oocytes. The channel blockers amantadine and barium, but not cesium, inhibit this conductance, in addition to virus plaque formation. Thus, PBCV-1 encodes the first known viral protein that functions as a potassium-selective channel and is essential in the virus Life cycle.
- Issue Date
- 2000
- Status
- published
- Publisher
- Amer Assoc Advancement Science
- Journal
- Science
- ISSN
- 0036-8075
- Sponsor
- NIGMS NIH HHS [GM32441, GM41333]; Telethon [971]