Orientation selection in distance measurements between nitroxide spin labels at 94 GHz EPR with variable dual frequency irradiation
2013 | journal article. A publication with affiliation to the University of Göttingen.
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Tkach, Igor, Soraya Pornsuwan, Claudia Höbartner, Falk Wachowius, Snorri Th. Sigurdsson, Tatiana Y. Baranova, Ulf Diederichsen, Giuseppe Sicoli, and Marina Bennati. "Orientation selection in distance measurements between nitroxide spin labels at 94 GHz EPR with variable dual frequency irradiation." Physical Chemistry Chemical Physics 15, no. 10 (2013): 3433-3437. https://doi.org/10.1039/c3cp44415e.
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Details
- Authors
- Tkach, Igor; Pornsuwan, Soraya ; Höbartner, Claudia ; Wachowius, Falk; Sigurdsson, Snorri Th.; Baranova, Tatiana Y.; Diederichsen, Ulf ; Sicoli, Giuseppe; Bennati, Marina
- Abstract
- Pulsed electron-electron double resonance (PELDOR, also known as DEER) has become a method of choice to measure distances in biomolecules. In this work we show how the performance of the method can be improved at high EPR frequencies (94 GHz) using variable dual frequency irradiation in a dual mode cavity in order to obtain enhanced resolution toward orientation selection. Dipolar evolution traces of a representative RNA duplex and an alpha-helical peptide were analysed in terms of possible bi-radical structures by considering the inherent ambiguity of symmetry-related solutions.
- Issue Date
- 2013
- Status
- published
- Publisher
- Royal Soc Chemistry
- Journal
- Physical Chemistry Chemical Physics
- ISSN
- 1463-9076
