Differential organization of tonic and chronic B cell antigen receptors in the plasma membrane
2019 | journal article. A publication with affiliation to the University of Göttingen.
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Gomes de Castro, Maria Angela, Hanna Wildhagen, Shama Sograte-Idrissi, Christoffer Hitzing, Mascha Binder, Martin Trepel, Niklas Engels, and Felipe Opazo. "Differential organization of tonic and chronic B cell antigen receptors in the plasma membrane." Nature Communications 10, no. 1 (2019): 820. https://doi.org/10.1038/s41467-019-08677-1.
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- Authors
- Gomes de Castro, Maria Angela ; Wildhagen, Hanna; Sograte-Idrissi, Shama; Hitzing, Christoffer; Binder, Mascha; Trepel, Martin; Engels, Niklas; Opazo, Felipe
- Abstract
- Stimulation of the B cell antigen receptor (BCR) triggers signaling pathways that promote the differentiation of B cells into plasma cells. Despite the pivotal function of BCR in B cell activation, the organization of the BCR on the surface of resting and antigen-activated B cells remains unclear. Here we show, using STED super-resolution microscopy, that IgM-containing BCRs exist predominantly as monomers and dimers in the plasma membrane of resting B cells, but form higher oligomeric clusters upon stimulation. By contrast, a chronic lymphocytic leukemia-derived BCR forms dimers and oligomers in the absence of a stimulus, but a single amino acid exchange reverts its organization to monomers in unstimulated B cells. Our super-resolution microscopy approach for quantitatively analyzing cell surface proteins may thus help reveal the nanoscale organization of immunoreceptors in various cell types.
- Issue Date
- 2019
- Journal
- Nature Communications
- ISSN
- 2041-1723
- Language
- English