Secondary Ion Mass Spectrometry Imaging Reveals Changes in the Lipid Structure of the Plasma Membranes of Hippocampal Neurons following Drugs Affecting Neuronal Activity

2021 | journal article; research paper. A publication with affiliation to the University of Göttingen.

Jump to:Cite & Linked | Documents & Media | Details | Version history

Cite this publication

​Agüi-Gonzalez, P., Bao, G., Gomes de Castro, M. A., Rizzoli, S. O. & Phan, N. T. N. (2021). ​Secondary Ion Mass Spectrometry Imaging Reveals Changes in the Lipid Structure of the Plasma Membranes of Hippocampal Neurons following Drugs Affecting Neuronal Activity. ACS Chemical Neuroscience12(9), ​1542​-1551​. ​doi: https://doi.org/10.1021/acschemneuro.1c00031 

Documents & Media

acschemneuro.1c00031.pdf10.32 MBAdobe PDF

License

Published Version

Attribution 4.0 CC BY 4.0

Details

Authors
Agüi-Gonzalez, Paola ; Bao, Guobin ; Gomes de Castro, Maria Angela ; Rizzoli, Silvio O. ; Phan, Nhu T. N. 
Abstract
The cellular functions of lipids in the neuronal plasma membranes have been increasingly acknowledged, particularly their association to neuronal processes and synaptic plasticity. However, the knowledge of their regulatory mechanisms in neuronal cells remains sparse. To address this, we investigated the lipid organization of the plasma membranes of hippocampal neurons in relation to neuronal activity using secondary ion mass spectrometry imaging. The neurons were treated with drugs, particularly tetrodotoxin (TTX) and bicuculline (BIC), to induce chronic activation and silencing. Distinct lipid organization was found in the plasma membrane of the cell body and the neurites. Moreover, significant alterations of the levels of the membrane lipids, especially ceramides, phosphatidylserines, phosphatidic acids, and triacylglycerols, were observed under the TTX and BIC treatments. We suggest that many types of membrane lipids are affected by, and may be involved in, the regulation of neuronal function.
Issue Date
2021
Journal
ACS Chemical Neuroscience 
Project
SFB 1286: Quantitative Synaptologie 
Working Group
RG Rizzoli (Quantitative Synaptology in Space and Time) 
External URL
https://sfb1286.uni-goettingen.de/literature/publications/119
ISSN
1948-7193
eISSN
1948-7193
Language
English

Reference

Citations


Social Media