Generation of Engineered Human Myocardium in a Multi-well Format
2020 | journal article; research paper
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Details
- Authors
- Tiburcy, Malte ; Meyer, Tim ; Liaw, Norman Y.; Zimmermann, Wolfram-Hubertus
- Abstract
- This protocol describes a robust method for the generation of engineered human myocardium (EHM) from pluripotent stem cells (PSCs) in a multi-well plate under defined, serum-free conditions. By parallel culture of up to 48 EHM in one plate, contractile heart muscle can be obtained to serve numerous applications, including drug screening and disease modelling. This protocol has been successfully applied to human embryonic stem (HES) cell- and induced PSC-derived cardiomyocytes, subtype-specific, i.e., atrial and ventricular, and commercially available cardiomyocyte preparations. For complete details on the use and execution of this protocol, please refer to Tiburcy et al. (2017).
- Issue Date
- 2020
- Journal
- STAR Protocols
- Project
- EXC 2067: Multiscale Bioimaging
SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz
SFB 1002 | S01: In vivo und in vitro Krankheitsmodelle - Working Group
- RG Zimmermann (Engineered Human Myocardium)
RG Tiburcy (Stem Cell Disease Modeling) - eISSN
- 2666-1667
- Language
- English