Intranuclear immunostaining-based FACS protocol from embryonic cortical tissue
2021 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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- Authors
- Sakib, M. Sadman; Sokpor, Godwin ; Nguyen, Huu Phuc; Fischer, André ; Tuoc, Tran
- Abstract
- Cell sorting can be used to purify cell populations for cell type-specific molecular probing. Fluorescence-activated cell sorting (FACS) coupled with high-throughput sequencing affords molecular signature identification for specific cell types. FACS has many challenges that limit comprehensive cell purification from the brain, leading to incomplete molecular characterization. Here, we present the intranuclear immunostaining-based FACS protocol with several modified steps, which allows optimized nuclei/cell sorting from mouse or human embryonic cortical tissue for distinct downstream molecular investigation of basal intermediate progenitors.
- Issue Date
- 2021
- Journal
- STAR Protocols
- Project
- EXC 2067: Multiscale Bioimaging
SFB 1286: Quantitative Synaptologie
SFB 1286 | B06: Die Rolle von RNA in Synapsenphysiologie und Neurodegeneration - Working Group
- RG A. Fischer (Epigenetics and Systems Medicine in Neurodegenerative Diseases)
- ISSN
- 2666-1667
- Language
- English