A presequence-binding groove in Tom70 supports import of Mdl1 into mitochondria
2015 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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A presequence-binding groove in Tom70 supports import of Mdl1 into mitochondria
Melin, J. ; Kilisch, M. ; Neumann, P. ; Lytovchenko, O. ; Gomkale, R.; Schendzielorz, A. B. & Schmidt, B. et al. (2015)
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1853(8) pp. 1850-1859. DOI: https://doi.org/10.1016/j.bbamcr.2015.04.021
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- Authors
- Melin, Jonathan ; Kilisch, Markus ; Neumann, Piotr ; Lytovchenko, Oleksandr ; Gomkale, Ridhima; Schendzielorz, Alexander Benjamin ; Schmidt, Bernhard ; Liepold, Thomas ; Ficner, Ralf ; Jahn, Olaf ; Rehling, Peter ; Schulz, Christian
- Abstract
- The translocase of the outer mitochondrial membrane (TOM complex) is the general entry gate into mitochondria for almost all imported proteins. A variety of specific receptors allow the TOM complex to recognize targeting signals of various precursor proteins that are transported along different import pathways. Aside from the well-characterized presequence receptors Tom20 and Tom22 a third TOM receptor, Tom70, binds proteins of the carrier family containing multiple transmembrane segments. Here we demonstrate that Tom70 directly binds to presequence peptides using a dedicated groove. A single point mutation in the cavity of this pocket (M551R) reduces the presequence binding affinity of Tom70 ten-fold and selectively impairs import of the presequence-containing precursor Mdl1 but not the ADP/ATP carrier (MC). Hence Tom70 contributes to the presequence import pathway by recognition of the targeting signal of the Mdl1 precursor. (C) 2015 Elsevier B.V. All rights reserved.
- Issue Date
- 2015
- Journal
- Biochimica et Biophysica Acta (BBA) - Molecular Cell Research
- ISSN
- 0167-4889
- eISSN
- 0006-3002
- Language
- English