Transcriptionally active enhancers in human cancer cells

2021 | journal article; research paper. A publication with affiliation to the University of Göttingen.

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​Lidschreiber, K., Jung, L. A, von der Emde, H., Dave, K., Taipale, J., Cramer, P. & Lidschreiber, M. (2021). ​Transcriptionally active enhancers in human cancer cells. Molecular Systems Biology17(1), Article e9873​. ​doi: https://doi.org/10.15252/msb.20209873 

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Authors
Lidschreiber, Katja; Jung, Lisa A; von der Emde, Henrik; Dave, Kashyap; Taipale, Jussi; Cramer, Patrick ; Lidschreiber, Michael
Abstract
The growth of human cancer cells is driven by aberrant enhancer and gene transcription activity. Here, we use transient transcriptome sequencing (TT-seq) to map thousands of transcriptionally active putative enhancers in fourteen human cancer cell lines covering seven types of cancer. These enhancers were associated with cell type-specific gene expression, enriched for genetic variants that predispose to cancer, and included functionally verified enhancers. Enhancer-promoter (E-P) pairing by correlation of transcription activity revealed ~ 40,000 putative E-P pairs, which were depleted for housekeeping genes and enriched for transcription factors, cancer-associated genes, and 3D conformational proximity. The cell type specificity and transcription activity of target genes increased with the number of paired putative enhancers. Our results represent a rich resource for future studies of gene regulation by enhancers and their role in driving cancerous cell growth.
Issue Date
2021
Journal
Molecular Systems Biology 
Project
EXC 2067: Multiscale Bioimaging 
Working Group
RG Cramer 
ISSN
1744-4292
Language
English

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