TRPV1 activation and internalization is part of the LPS-induced inflammation in human iPSC-derived cardiomyocytes
2021 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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TRPV1 activation and internalization is part of the LPS-induced inflammation in human iPSC-derived cardiomyocytes
Sattler, K.; El-Battrawy, I.; Cyganek, L. ; Lang, S.; Lan, H.; Li, X. & Zhao, Z. et al. (2021)
Scientific Reports, 11(1) art. 14689. DOI: https://doi.org/10.1038/s41598-021-93958-3
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Details
- Authors
- Sattler, Katherine; El-Battrawy, Ibrahim; Cyganek, Lukas ; Lang, Siegfried; Lan, Huan; Li, Xin; Zhao, Zhihan; Utikal, Jochen; Wieland, Thomas; Borggrefe, Martin; Akin, Ibrahim
- Abstract
- Abstract The non-selective cation channel transient receptor potential vanilloid 1 (TRPV1) is expressed throughout the cardiovascular system. Recent evidence shows a role for TRPV1 in inflammatory processes. The role of TRPV1 for myocardial inflammation has not been established yet. Human induced pluripotent stem cell (iPSC)-derived cardiomyocytes (hiPSC-CM) from 4 healthy donors were incubated with lipopolysaccharides (LPS, 6 h), TRPV1 agonist capsaicin (CAP, 20 min) or the antagonist capsazepine (CPZ, 20 min). TRPV1 expression was studied by PCR and western blotting. TRPV1 internalization was analyzed by immunofluorescence. Interleukin-6 (IL-6) secretion and phosphorylation of JNK, p38 and ERK were determined by ELISA. TRPV1-associated ion channel current was measured by patch clamp. TRPV1-mRNA and -protein were expressed in hiPSC-CM. TRPV1 was localized in the plasma membrane. LPS significantly increased secretion of IL-6 by 2.3-fold, which was prevented by pre-incubation with CPZ. LPS induced TRPV1 internalization. Phosphorylation levels of ERK, p38 or JNK were not altered by TRPV1 stimulation or inhibition. LPS and IL-6 significantly lowered TRPV1-mediated ion channel current. TRPV1 mediates the LPS-induced inflammation in cardiomyocytes, associated with changes of cellular electrophysiology. LPS-induced inflammation results in TRPV1 internalization. Further studies have to examine the underlying pathways and the clinical relevance of these findings.
- Issue Date
- 2021
- Journal
- Scientific Reports
- Project
- SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz
SFB 1002 | S01: In vivo und in vitro Krankheitsmodelle - Working Group
- RG Cyganek (Stem Cell Unit)
- eISSN
- 2045-2322
- Language
- English